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Tnimage is a powerful and flexible scientific image analysis
program for the Unix platform. It has been extensively used and
tested by molecular biologists, forensic pathologists, biochemists,
physicists, and others to obtain quantitative data from images.
It is also very useful for general image viewing and editing.
The program has an easy to use, menu-driven interface based on
Motif. Precompiled binaries are available for several Unix
platforms and MS-DOS.
This is a significant upgrade from the 2.5.x versions.
Version 2.6.5 of tnimage is now available by ftp at the following locations:
sunsite.unc.edu/pub/Linux/apps/graphics/misc/tnimage-2.6.5e.linux.tar.gz
sunsite.unc.edu/pub/Linux/apps/graphics/misc/tnimage-2.6.5e.linux.static.tar.gz
las1.ninds.nih.gov/pub/unix/tnimage-2.6.5e.linux.tar.gz
las1.ninds.nih.gov/pub/unix/tnimage-2.6.5e.linux.static.tar.gz
las1.ninds.nih.gov/pub/unix/tnimage-2.6.5e.irix.tar.gz
las1.ninds.nih.gov/pub/unix/tnimage-2.6.5e.linux.lesstif.tar.gz
las1.ninds.nih.gov/pub/unix/tnimage-2.6.5e.solaris.tar.gz
las1.ninds.nih.gov/pub/unix/tnimage-2.6.5e.src.tar.gz
las1.ninds.nih.gov/pub/unix/tnimage-2.6.5e.dvi.tar.gz
las1.ninds.nih.gov/pub/unix/tnimage-2.6.5e.manual.ps.gz
las1.ninds.nih.gov/pub/unix/tnimage-2.6.5e.manual.html.tar.gz
The DOS version (2.1.6) is available from:
las1.ninds.nih.gov/pub/dos/tnimg216.zip
The correct file sizes should be:
1692878 tnimage-2.6.5e.irix.tar.gz
2059936 tnimage-2.6.5e.linux.lesstif.tar.gz
2505459 tnimage-2.6.5e.linux.static.tar.gz
1567912 tnimage-2.6.5e.linux.tar.gz
328644 tnimage-2.6.5e.manual.dvi.tar.gz
1014649 tnimage-2.6.5e.manual.html.tar.gz
656722 tnimage-2.6.5e.manual.ps.gz
1592422 tnimage-2.6.5e.solaris.tar.gz
1187347 tnimage-2.6.5e.src.tar.gz
477423 tnimg216.zip
Summary :
---------
o Scientific image analysis program for the X Window System.
o Mouse & menu-based image editing and analysis with user-friendly
interface.
o Includes 137-page manual, tutorials, and on-line help.
o Highly customizable.
System requirements:
--------------------
o Unix version requires X11R5 or higher and Motif 1.2 or higher.
Statically-linked version does not require Motif.
o Binaries are provided for the Linux (x86), Solaris, Irix,
ConvexOS, and MS-DOS.
o DOS version requires SVGA card and 4 MB of RAM; handles all
VESA screen modes including 1600x1200, as well as XGA and XGA-2
video cards.
New features since version 2.5.0:
--------------------------------
o Moveable text labels. Uses antialiasing in rotated labels.
Alpha channel can be viewed separately.
o Image and label rotation (granularity=0.0001 degrees) with adjustable
spacing. "Label" option facilitates positioning of labels.
o Image algebra allows user-defined mathematical transformations of
the image using C-like syntax.
o Non-rectangular areas can now be selected. "Smart" object selection
automatically identifies and selects objects in image when mouse is
double-clicked.
o Area copy can now be done by clicking on a selected area and dragging
the selection to a new location. The "Copy" and "Move" buttons have
been removed.
o Integrated scanner control; HP SCSI scanner driver built in, other
scanners and cameras are controlled with external drivers.
Preview scan and interactive image scanning at 8, 10, 12, 24, 30,
and 36 bits/pixel (Not available in ConvexOS and MS-DOS versions).
Sample configuration file for Umax scanners is included.
o Zooming of images and background by an arbitrary factor. All image
editing features, including area copy, labels, filtering, and FFT
also work on zoomed images.
o Image transparency and chromakey for producing composite images.
o Spreadsheet display for images. Images can be viewed directly or in
an editable spreadsheet using integers, RGB values, hex integers, or
(if an image has been FFT'd) floating point numbers. Changes in the
image are immediately reflected in the spreadsheet and vice-versa.
o Spot densitometry uses fuzzy k-means algorithm to automatically and
accurately determine the background and foreground values for each
measurement. This produces far more accurate results than other
methods and obviates the need to estimate the background value manually.
o Complete rewrite of manual with example images. Manual is available
in HTML, dvi, and PostScript format.
o Reorganized information area window to display more information.
o Arrow drawing, similar to Photoshop arrow mode, added.
o The "Macro" window executes each line as a command when <Shift-Enter>
is pressed. This allows it to be used as a command-line interface
instead of having to use the menus.
o Variable-width lines added. Wide lines can have color gradient and
skew for 3D effect.
o Improved and simplified gradient fill interface.
o Images can be split into red, green, and blue subimages and recombined.
o "Spray math", "spray erase", and "spray filtering" functions allow
any mathematical formula or filter to be applied interactively to
part of the image as a spray, or changes to be partially or completely
undone.
o Users can easily create new custom buttons on the main window.
The buttons can execute any command or macro.
o Gamma tables, colormaps, and image calibration parameters can now be
copied from one image to another.
o Multi-frame (3D) images can now be played back as movies.
o Plug-in support. Users can easily add new image analysis
features with a few lines of code.
o Image selection and image property windows are automatically updated
when any property of the image is changed.
o Data on disk can now be imported into graphs.
o Several bug fixes and performance enhancements. Many other small
improvements have also been made.
o Installation script and man page added.
Image analysis features:
------------------------
o Densitometry of rectangular regions or arbitrary-shaped areas.
Edges of spots can be determined automatically. Results can be easily
exported to disk in ASCII format.
o Strip (scanning) densitometry of transepts, fixed-width rectangular
regions, and trapezoidal areas. This is particularly useful for
densitometry of SDS and agarose gels. Can automatically subtract
baseline and integrate peak areas, or interactively measure peaks
with the mouse. Peak area table can be saved in ASCII format.
o 2D-Fourier transform. Real, imaginary, or power spectra can be viewed
and edited.
o FFT frequency components can be changed using the drawing or image
algegra functions or exported in ASCII format for manual editing
of specific frequencies, imported, and transformed back to the real
image. A tutorial is provided for digital filtering using the FFT.
o Convolution of 2 images and image reconstruction using Fourier
deconvolution.
o Distance and angle measurement.
o Image algebra function allows multiple images to be subtracted or
otherwise transformed according to arbitrary user-defined equations.
o Curve tracing.
o Calibration of X-Y coordinates using linear, polynomial, or exponential
functions. For example, the logarithm of image coordinates in one
direction can be expressed as molecular weight.
Other features:
--------------
o Create, cut/paste, and add text labels in multiple fonts and graphic
elements such as circles, Bezier curves, freehand drawing, etc.
o Handles up to 512 images of any depth simultaneously. Each image can
be in a separate window or in a single large window to facilitate
creation of composite images.
o Copy and paste seamlessly between images of different pixel depths,
even if images are in different windows.
o Prints to PCL or PostScript printer. CMY, CMYK, or RGB formats.
o Image formats: PCX, IMG, TIFF, JPEG, BMP, GIF, TGA, IMG, PBM, PGM,
PPM, XWD, FITS, PDS, IMDS, Lumisys Xray scanner, and ASCII images,
of any depth from 1-32 bits per pixel, color or monochrome, raw binary
images, multi-frame 3D images (such as PET scan and confocal images),
and user-definable image formats. Handles unusual image depths such as
12- and 17-bit grayscale that are unreadable in other programs.
Additional formats can be added using plugins or defined interactively.
o Interconversion of image formats and image types.
o Solid and gradient flood fill.
o R, G, and B image planes can be manipulated separately.
o Adjust color, intensity, contrast, and grayscale mapping. Grayscale
images deeper than 8 bits/pixel, such as medical grayscale images,
can be viewed with a sliding scale to enhance any particular intensity
region.
o Rotate, resize, warp, flip image; invert or remap colors; crop, paint,
spray paint, etc.
o Interactively create arbitrary colormaps or select from 10,000
pre-defined colormaps.
o Convolution filters: sharpen, blur, edge enhancement, shadow
sharpening, background subtract, background flatten, noise filter,
Sobel, and Laplace filtering.
o Macro language and macro editor. Macro programming guide is included.
o RGB & intensity histograms.
o 3D images can be viewed interactively as a movie, or each frame can
be manipulated separately.
o Color to grayscale conversion, change image depths,
Distribution policy:
--------------------
Freely distributable.
For additional information:
---------------------------
tjnelson _at_ las1.ninds.nih.gov
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